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''I can thoroughly recommend this book as being especiallyhelpful to those involved in biological, medical, chemical, or fiberresearch,or indeed any environment involving microscopies, beingqualitative,thorough, and up-to-date, but not too technical in itsapproach.''--Optica Acta, from a review of the FirstEditionThe authors have completely revised and updated their work toaccount for new technologies and techniques. ... Read more

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Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work.The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data.Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss.Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.

* Updates the numerous technological innovations that have improved the capabilities of electron microscopy
* Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging
* Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going ... Read more

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The first practical guide to the maintenance of electron microscopes, this book enables operators to keep the instrument in optimum condition, analyze its performance, and locate and correct faults.To facilitate mastery of these procedures, the author includes a description of high resolution operating techniques and examples of typical faults, as well as advice on when a problem requires a service engineer. This straightforward and thorough handbook will help any operator to maximize his or her use of the microscope without having to rely on pre-set controls or conventional microscopy teaching. ... Read more

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This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures. ... Read more

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Provides a practical introduction to the use of scanning electron microscopy (SEM). Covers sample preparation, instrumentation and the more complex area of SEM-X-Ray Microanalysis. Mathematics are kept to a minimum with the emphasis on developing an understanding of the practical aspects of the techniques. The use of self-assessment questions to reinforce the learning process is particularly valuable. ... Read more

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The electron microscope has been used in hair research for half a century, but the knowledge gained through this study has become rather fragmented over the years. While the molecular biological study of hair has become an active field, even here, as in more traditional research, an understanding of the morphological aspects is essential because the hair follicle structure is highly complex. The author explicates the structure, development, and differentiation of the hair follicle, using his own original photographs and explanations of current research, including growth factors, differentiation-inducers, cell signaling pathways, and transcription factors. With neonatal rats as subjects, he focuses on the morphological analysis of all basic hair cells - in the shaft, matrix, and papilla - from birth to death. The introductory chapter will benefit novices, with the following sections consisting mainly of explanations of the electron microscopic photographs of hair cells.

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This book is a comprehensive review of the theories, techniques and applications of reflection electron microscopy (REM), reflection high-energy electron diffraction (RHEED) and reflection electron energy-loss spectroscopy (REELS). The book is divided into three parts: diffraction, imaging and spectroscopy.The text is written to combine basic techniques with special applications, theories with experiments, and the basic physics with materials science, so that a full picture of RHEED and REM emerges. An entirely self-contained study, the book contains much invaluable reference material, including FORTRAN source codes for calculating crystal structures data and electron energy-loss spectra in different scattering geometries. This and many other features makes the book an important and timely addition to the materials science literature for researchers and graduate students in physics and materials science. ... Read more

Customer Reviews (2)

From: Analysis, 1997
The book describes the analytical techniques based on electrondiffraction, reflection and imaging in the TEM (and STEM) for the analysisof materials surfaces. As with most texts dealing with analyticaltechniques these days it is awash with acronyms and the ones representingthe candidate methodologies are frequently encountered. These are RHEED(reflection high energy electron diffraction), REM (reflection electronmicroscopy), SREM (scanning REM) and REELS (reflection electron energy lossspectroscopy). Many others are included, but the author is considerateenough to define them all in the early pages of his book. The dust-covernotes state that this is 'an entirely self-contained study' in which the'theories, techniques and applications of REM, RHEED and REELS arecomprehensively reviewed'. Inspection of the text reveals that this is,indeed, the case, with three parts (logically Part A, Part B and Part C) ofapproximately equal length covering the three areas of reflection electronstudies. This is preceded by a comprehensive review of the kinematicaltheory of electron diffraction. This chapter is a very helpful (andnecessary) prelude to the rest of the book. It deals with kinematicalscattering in the usual numerical manner but the associated text makes thischapter extremely readable. The main body of the text is written in anequally attractive style with the theory of the various topics beingintroduced alongside the experimental procedures involved. There are manyapplications of reflection electron microscopy and spectroscopy scatteredthroughout the book; although inmost cases they serve as illustrations ofparticular facets of the experimental procedures rather than points whichemphasize the relative strengths of the candidate techniques. This is,however, a minor criticism, and the inclusion of examples of realmicrographs, diffraction patterns and spectra throughout the text mayprovide some readers with much needed relief from the undoubted rigour ofthe theoretical treatments provided.

For those with a TEM background itrepresents, perhaps, the definitive text for reflection methods.

Thereare two particularly attractive aspects of this book to be found in theclosing pages. The first is an extensive set (ten) of appendices whichcontain much useful data along with five FORTRAM programs for interpretingspectra and modeling electron beam/specimen interaction. These havepresumably been widely tested in the author's laboratory and theirinclusion here is to be welcomed. The other feature, warmly welcomed bythis reviewer, is the inclusion of a separate index of the materials usedto illustrate the various facets of the reflection techniques. Alsoincluded as an Appendix is a chronological bibliography of REM, SREM andREELS covering the years 1975-1995. RHEED is presumably excluded as it isthe most senior, and widely used, of the methods considered. This book isnot one for those with a peripheral interest in RHEED, REM, SREM and REELS.Referring once again to the cover notes it is offered as an 'ideal guidefor scientists and graduate students working on quantitative surfacestructure characterization using reflection electron techniques' and thereis no doubt that this target audience will appreciate the publication ofsuch a concise, authoritative and well written text in their chosen area ofendeavor. For those with a TEM background it represents, perhaps, thedefinitive text for reflection methods and provides all the theoreticalinformation necessary for a thorough appreciation of these techniques. Atsuch a reasonable price for a very specialist text one would hope that itwill soon find a place on the bookshelf of every electron microscopy unitwith a practical need (or even aspirations) to carry out surface structuredetermination in the TEM or STEM. For those with a need for such a textthis book fulfills all the claims made on its behalf. Dr. Wang is to becongratulated on writing a very accessible text. The book is thoroughlyrecommended.

A book you must have
"It contains a lot of illustrations and excellent images and a goodbalance of theory and experimental techniques... it is a book that anymaterials science or physics libraries should be holding" ... Read more

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1969 dual language book (French and English) describing and illustrating biological cellular structure and disease. Extensive photographic detail using both electron and regular light microscopes. Volume 1 of a two volume set. ... Read more

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This "hands-on" laboratory guide provides full coverage of the principles and operation of both transmission and scanning electron microscopes.Readers will find a useful explanation of how and why electron microscopes work, as well as information on the electron gun, electromagnetic lenses, electron detection, and the vacuum system.The operation and alignment of the two types of microscopes is covered in separate chapters, while clear, detailed instructions help the novice to produce successful micrographs.The book also furnishes advice on how to optimize the accelerating voltage, condenser lens settings, aperture size and magnification, and on photographic recording.It will be of value to researchers in a wide variety of fields, including biology, medicine, physics, materials science, and engineering. ... Read more

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As a complement to The Beginnings of Electron Microscopy, Advances in Imaging and Electron Physics is pleased to present Volume 96, The Growth of Electron Microscopy. This comprehensive collection of articles surveys the accomplishments of various national groups that comprise the International Federation of Societies of Electron Microscopy (IFSEM). ... Read more

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While most textbooks about scanning electron microscopy (SEM) cover the high-voltage range from 5-50 keV, this volume considers the special problems in low-voltage SEM and summarizes the differences between LVSEM and conventional SEM. Chapters cover the influence of lens aberrations and design on electron-probe formation; the effect of elastic and inelastic scattering processes on electron diffusion and electron range; charging and radiation damage effects; the dependence of SE yield and the backscattering coefficient on electron energy, surface tilt, and material as well as the angular and energy distributions; and types of image contrast and the differences between LVSEM and conventional SEM modes due to the influence of electron-specimen interactions.

Contents:
- Introduction
- Electron Optics and Instrumentation
- Electron Scattering and Diffusion
- Backscattered and Secondary-Electron Emission
- Specimen Charging and Damage
- Signal Formation and Linage Contrast
- Electron Spectroscopic Methods ... Read more

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Stedings and Virághs Scanning Electron Microscopy Atlas of the Developing Human Heart comprises a complete and extensive exposure of the spatial and temporal aspects of human cardiac development as seen with scanning electron microscopy. Apart from serving as a unique overview on cardiac development in the human embryo, this atlas gives an updated morphological reference of cardiac embryology for topographic correlation and enables the projection of experimental results in animals to the human situation.