Product Description
Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries.

Copy for Both Volumes

Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function. ... Read more

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California Institute of Technology, Pasadena. Text presents experimental protocols for the generation of molecular diversity. Includes reproducible methods for random mutagenesis of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria. For researchers. ... Read more

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This landmark volume provides the single most comprehensive and authoritative textbook on bacterial molecular genetics.Perfect for advanced undergraduate and graduate-level courses, the text presents the latest research on the subject in a clearly written and well-illustrated style.It provides descriptive background information, detailed experimental methods, examples of genetic analyses, and advanced material relevant to current applications of molecular genetics.While providing a deep understanding of bacterial molecular genetics, the material is integrated with biochemical, genomic, and structural information to broaden understanding.

The approach centers on the most-studied bacteria, Escherichia coli and Bacillus subtilis. In addition, examples from other bacteria with medical, ecological, or biotechnological significance are covered throughout the text.The material in each chapter has been substantially revised and rewritten and reflects exciting developments in the field of bacterial molecular genetics and its relationship to other fields, including genetics, biotechnology, and bioengineering.

Molecular Genetics of Bacteria, 3rd Edition is organized like the two previous editions. Each chapter contains a summary of main points, descriptions of significant experiments, a set of discussion questions and a problem set (with answers to both at the back of the book), and a list of suggested readings--all updated to reflect the most recent advances in the field.Additionally, text boxes present intriguing information on each topic without interrupting the continuity of the text.

This edition will be invaluable to anyone working in the fields of microbiology, genetics, biochemistry, bioengineering, medicine, molecular biology, and biotechnology. It is also essential reading for scientists in all fields of biology, many of whom depend upon the information in this book.

New in the Third Edition
* Completely revised and updated material, incorporating the most recent advances in the field
* Introduces over 100 brand-new illustrations
* Offers a revised and expanded glossary containing approximately 1,000 entries ... Read more

Customer Reviews (12)

Slow Shipping
The product I bought, when if finally came was in good shape.I have no complate about the condition the book was in when it finally came.However, it took nearly a month for the book to be shipped to me.

where's the money about the first order I made.
I ordered twice of this book, because the first order was delivered, and the money was not refund to my account even now!!

the second one is good, they delivered another one for me.


but I am really curious about, where's my money for the first one I ordered?!!,
will you hurry up to refund my money back?!! it has already one momth past

delivered as promised
It was delivered in the time specified and the item was in flawless condition.I would definitely use this seller again.

Great
I had a great experience--the book is in great condition and I received it in a timely manner.

the book condition is excellent, but it took so long to get.
The book condiotion is very good. However, it took almost 1 month for the product to arrive. ... Read more

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With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.

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Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena. ... Read more

Product Description
Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful application of a technique?
* Are you familiar with the limitations of a given technique?
* When should common procedural rules of thumb not be applied?
* What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
* Working safely with biological samples and radioactive materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment ... Read more

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A comprehensive multidisciplinary review of the most relevant molecular, genetic, and behavioral approaches used to investigate the neurobiological basis of drug addiction. The authors explore the latest findings on opioid, psychostimulant, cannabinoid, alcohol, and nicotine addiction, provide fresh insights into the genetic basis of drug addiction and the new therapeutic perspectives these have opened. They describe the technology available to generate conditional knockout mice and show how these mice can reveal the molecular basis of opioid, psychostimulant, and cannabinoid addiction. They also review the different behavioral models available to evaluate the rewarding effects of drugs and analyze the genes involved in alcohol dependence. ... Read more

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The latest volume in this series covers recent research in molecular biology and biochemistry.It provides information on such rapidly expanding areas as manipulatingmonoclonal antibodies, diseases of abnormal antigen receptor formation, and the molecular mechanisms of autoimmunity.It will be useful to researchers in molecular biology, immunology, and biochemistry who need to keep up with new developments in this field. ... Read more

Product Description
In the Dictionary of Plant Genetics and Molecular Biology, more than 3,500 technical terms from the fields of plant genetics and molecular biology are defined for students, teachers, and researchers in universities, institutes, and agricultural research stations. An excellent educational tool that will save you time and effort, this dictionary brings together into a single source the meaning and origin of terms from the fields of classical genetics, molecular genetics, mutagenesis, population genetics, statistics, plant biotechnology, evolutionary genetics, plant breeding, and plant biotechnology.

Finding and understanding the precise meaning of many terms in genetics is crucial to understanding the foundation of the subject matter. For reasons of space, the glossaries provided at the end of most textbooks are highly inadequate. There is, then, dire need for a dictionary of terms in a single volume. You?ll appreciate the helpful approaches and features of Dictionary of Plant Genetics and Molecular Biology, including:

• no terms that are of limited use, very general, or self-explanatory
• cross references for effective access to the materials and economy of space
• alternate names of terms, denoted with “Also referred to as . . .” or “Also known as . . .”
• multiple definitions for terms defined by different authors or for terms with different meanings in different contexts
• authors who coined, described, or contributed toward further understanding of a term are listed and respective publications are included in the Bibliography
  
  At last, there is compiled in a single volume the technical terms you need to know in order to understand plant genetics and molecular biology. As your knowledge grows, you?ll uncover even more terms that you need to understand. You?ll find yourself turning to this handy guide time and time again for help on all levels.

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While there is a wide selection of 'by experts, for experts’ books in statistics and molecular biology, there is a distinct need for a book that presents the basic principles of proper statistical analyses and progresses to more advanced statistical methods in response to rapidly developing technologies and methodologies in the field of molecular biology. Statistical Methods in Molecular Biology strives to fill that gap by covering basic and intermediate statistics that are useful for classical molecular biology settings and advanced statistical techniques that can be used to help solve problems commonly encountered in modern molecular biology studies, such as supervised and unsupervised learning, hidden Markov models, methods for manipulation and analysis of high-throughput microarray and proteomic data, and methods for the synthesis of the available evidences. This detailed volume offers molecular biologists a book in a progressive style where basic statistical methods are introduced and gradually elevated to an intermediate level, while providing statisticians knowledge of various biological data generated from the field of molecular biology, the types of questions of interest to molecular biologists, and the state-of-the-art statistical approaches to analyzing the data. As a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of meticulous descriptions and implementation advice for diverse topics that are crucial for getting optimal results.

Comprehensive but convenient, Statistical Methods in Molecular Biology will aid students, scientists, and researchers along the pathway from beginning strategies to a deeper understanding of these vital systems of data analysis and interpretation within one concise volume.

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A Guide to the Fundamentals and Latest Concepts of Molecular and Cell Biology

Bridging the gap between biology and engineering, Applied Cell and Molecular Biology for Engineers uses clear, straightforward language to introduce you to the cutting-edge concepts of molecular and cell biology.

Written by an international team of engineers and life scientists, this vital tool contains “clinical focus boxes” and “applications boxes” in each chapter to link biology and engineering in today's world. To help grasp complex material quickly and easily, a glossary is provided.

Applied Cell and Molecular Biology for Engineers features:

• Clear descriptions of cell structures and functions
• Detailed coverage of cellular communication
• In-depth information on cellular energy conversion
• Concise facts on information flow across generations
• A succinct guide to the evolution of cells to organisms

Inside This Biomedical Engineering Guide

Customer Reviews (1)

Excellent book on a very complex subject
PETER TEIMAN FRANKLIN here,
Superb introduction to bioengineering. Whilst not in-depth, it certainly covers the important topics.
PETER TEIMAN FRANKLIN,
Sweden
... Read more

Product Description
The second edition retains all the beauty of the first, and it serves as an editorial model -- a guide that shows us how serious authors prepare a new version of a serious book.

The first edition was the work of seven authors, all of them outstanding scientists [note 2]. For the second edition, the team of authors has been expanded to include a science writer, Karen Hopkin, whose main responsibility (the Preface tells us) has been "to make the book clear, accessible, and fun to read."

As in the earlier edition, there is a digest of "Essential Concepts" near the end of each chapter, and the illustrations are grouped into panels that actually explain principles, structures, procedures and experiments, rather than just providing pretty distractions. Some of the material has been reordered, the coverage has been broadened to include some new findings and new methods, there is more emphasis on genetics, and there is an expanded, improved complement of questions that make students take time to think, to realize that a question often has more than one valid answer, and to recognize that alternative perspectives are available in almost any situation. All of these changes are welcome.

...There are 21 "How We Know" articles -- one in every chapter -- providing information about metabolic pathways, protein structures, replication, the genetic code, mitosis, and cancer, among other topics. These articles… [are] very important innovations....Each copy of the new edition is accompanied by a CD-ROM, and the CD-ROM is not a mere gimmick or toy. It is a valuable adjunct to the textbook, and it has two important functions. First, it carries the literature references for each chapter in the book. (The references don't appear in the book itself.) Second, the CD-ROM provides interactive illustrations, typically with voice-over narrations, that demonstrate such things as the structures of molecules, the movement of cells, and the processes of replication, translation and cell division.

What makes for a great science education is not the amassing of facts but the understanding of concepts, processes and syntheses, and the realization that all our information about nature must be subject to testing and revision. That is the kind of educational experience that students can acquire from the second edition of Essential Cell Biology.

I recommend this new edition for use in advanced high-school courses aimed at students who already have had a course in chemistry and a solid introductory course in biology. This fine book also belongs in the library of any high school that offers serious instruction in science. Teachers and librarians who did not get the first edition should look at this second edition pronto.

from The Textbook Letter, Volume 12, Number 2, 2005 ... Read more

Customer Reviews (46)

Intro to Cell Biology
There is not a better introductory book. I do not have a background in Biology except what I had in High School and I can follow this.

Book Review
I like this book because I have used it before for a previous Biology class. I wanted to purchase it again for future reference.

Ok
The book came in the condition that was described. Unfortunately it did not arrive until two weeks later even though I paid expedited shipping costs. It was really an inconvenience because I needed the book to study for a test in my class.

Essential Cell Biology
Very good cell and molecular biology book. It is in-depth and specific. The only problem is the uninviting font.

Great service, great book!
The book arrived really quickly and was in great condition - exactly as expected. Thanks so much! ... Read more

Product Description
This hugely influential book, published in 1966 as a 60th birthday tribute to Max Delbruck, is now republished as The Centennial Edition. On first publication, the book was hailed as ''[introducing] into the literature of science, for the first time, a self conscious historical element in which the participants in scientific discovery engage in writing their own chronicle. As such, it is an important document in the history of biology... '' (Journal of History of Biology). And in another review it was described as ''required reading for every student of experimental biology...[who] will sense the smell and rattle of the laboratory'' (Bioscience). The book was a formative influence on many of today's leading scientists. Related Titles from the Publisher Phage and the Origins of Molecular Biology (Expanded Edition); Phage and the Origins of Molecular Biology (First Edition); The Eighth Day of Creation: The Makers of the Revolution in Biology; Inspiring Science: Jim Watson and the Age of DNA; The Statue Within: An Autobiography; George Beadle, An Uncommon Farmer: The Emergence of Genetics in the 20th Century; Conversations in Genetics: An Oral History of Our Intellectual Heritage in Genetics; A Passion for DNA: Genes, Genomes, and Society; We Can Sleep Later: Alfred D. Hershey and the Origins of Molecular Biology ... Read more

Customer Reviews (1)

On the shoulders of Giants
This book recreates the times where an intimately connected group of brilliant young scientits came togeteher to do research around the inspiring and challenging Max Delbruck. What mattered above all was the science.And everything fell in place: Cold Spring Harbor, Cambridge, Institut Pasteur and Caltech. What came out is Molecular Biology, no more, no less... Read this book to understand that science like this can only happen once and the atmosphere around it can never be duplicated. ... Read more

Product Description
A diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods for studying B-lymphocyte development and function in higher animals. These readily reproducible protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. ... Read more

Product Description
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using todays biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. ... Read more

Customer Reviews (16)

Great reference book
For all biology scientists, this is a great buy and price is a bargain.Came in great condition.

Molecular Cloning Manual
The books arrived in good condition, but it took a long time to arrive here.

Molecular cloning: a lab Manual
This manual is high quality for the study of biotechnology. The authors collected lot of protocols and provided more detail of principle than previous two editions. The third edition involved three volumes. The manual is very useful for lab researcher.

Molecular Cloning: A Laboratory Manual (3-Volume Set)
This revised version of the Standard Lab Handbook has been improved, completed with new techniques and search is facilitated by the indices added at the end of each volume.

a formerly essential classic
For many years the previous edition of this set was an essential reference in molecular biology labs.At present however, there are too many good protocol books out there to really make this argument.The book is pretty strong in explaining theory, and answering the question of why certain procedures are either necessary, useful, or worthless, however it is not as practical as many other books, such as Short Protocols.Still a good reference overall, but no longer stands alone, and I recommend checking out as much of the competition as possible before deciding whether to make the investment in it. ... Read more

Product Description
In Silico introduces Maya programming into one of the most fascinating application areas of 3D graphics: biological visualization. In five building-block tutorials, this book prepares animators to work with visualization problems in cell biology. The book assumes no deep knowledge of cell biology nor 3D graphics programming. An accompanying DVD-ROM includes code derived from the tutorials, the working Maya computer files, and sample animated movies. The interior of the book contains generous Maya screen captures, tables, and flowcharts to compliment the instruction and tutorials. Full 4 color illustrations rendered from the authors' working files illustrate the capacity to produce striking pictures from the rendered models being created. Throughout the text, key words are set in bold type and subsequently defined in a glossary. Page margin tips and tricks compliment the adjacent copy, and icons direct the reader to specific references within the Maya Help library. The visualizations in the book are stunning examples of the state of the art.

*Teaches artists and scientists to create realistic digital images of humans and nature with popular CG program, Maya

*This self-contained study guide includes background, foundations, and practice

*Step-by-step example programs and end-result demonstrations help readers develop thie own portfolios

*Gorgeous four color screen shots throughout ... Read more

Customer Reviews (2)

Excellent resource
Firstly, let's talk about the structure of the book. This book is broken into three sections:

PART 1 is an introduction to the evolution of computing and how CG has been used for medical simulations and scientific visualizations. It contains three chapters that discuss how hardware and software are progressing over the years in respect of technology. So, this first part of the book is a bit overwhelming on theory. Those who are familiar with CG concepts and terms might see interesting to skip this part.

PART 2 is a Maya crash course. Well, not so "crash" if you consider this is a ten chapters walk. It goes from Maya Interface through Dynamics ending up with two great MEL scripting chapters. Maya users may want to skip this part. However, I consider the two chapters on MEL scripting valuable even for those who are already Maya/MEL users.

PART 3 is the heart of the book. However, it is a rock-solid one. This part contains five project-based chapters. If you are an experienced Maya and MEL user, so you are at one of the most interesting playgrounds you could be. If you are not experienced with Maya/MEL, I suggest you to go back to PART 2 and consider reading it. These five projects/tutorials will make you dive deep into the world of MEL scripting. And believe me: it's rewarding.

This is not a book for the casual 3D modeler and animator. This one is for those really interested in how Maya and MEL can be used to generate 3D animations and simulations for the biology and medical fields. Almost every picture inside this book is related with biomedical applications.

Those looking for learning Maya, this book really brings a pretty descent foundation course on this software. Those looking for learning MEL, this book is a must buy. Just please keep in mind that every example and exercise is targeted to cell biology and its related fields.

Best regards,
Chris.

this book will get you thinking
Overall I think this book is a decent addition to anyone looking at science visualization if you have a good knowledge of Maya already.
The book is broken into two sections. The first half of the book is really just an intro to maya. If you need this info, then the second half will completely blow you out of the water. I found the second half to be fairly engaging- it is really an exercise in MEL scripting - and a good one.
I think the book could give a more general overview of sci-viz, and not get into specifics so much.
This book is not for the casual Maya user. ... Read more

Product Description
With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances.This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine.Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry.As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. ... Read more

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The organizational history of Caltech is the loom on which Kay has woven an intricate fabric of the molecular vision of life. ... Read more

Customer Reviews (1)

Rockefeller's Vision
The information in this book is astonishing. A summary can only sell the book short, but here goes it.The Rockefeller family was hated for lack of business ethics, so they hired a PR man to improve their image.He recommended they give to a worthy cause like improving the health of people or something like that.What they did was form a committee to research what it would take to reform the practices of western medicine.It was determined that buying a relatively small number of the largest medical institutions and controlling what was taught to medical students, they could control how medicine was practiced in America.Well he bought up some medical schools, closed the rest, and effectively removed nutrition, and holistic and herbal medicine from the curriculum, and replaced it with a doctrine of pharmaceuticals and surgery.Of course he owned major shares in the pharmaceutical producing companies and could only profit from this.So instead of doing something that would improve their family image, they improved their family image and began to prey on the ill. I hung on every word in this book and the information it contains is invaluable.I wish everyone could read it.

I'd also like to make a side point.Successful industries create their market, so what do you do if you need to sell drugs to make a profit?You create sick people.How do you do that?You promote unhealthful practices, cure nothing, and make sure doctors only treat symptoms.You also lobby to make it mandatory for children to take vaccines containing aluminum, mercury, cancer causing viruses, foreign DNA, etc., etc. resulting in chronic disease and allergies.That's how, and that is just for starters.Believe it or not, that is what was and is being done to us, and then some.Kind of makes you sick, doesn't it? ... Read more

Product Description

Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fourth edition of the Handbook of Biochemistry and Molecular Biology represents a dramatic revision — the first in two decades — of one of biochemistry's most referenced works. This edition gathers a wealth of information not easily obtained, including information not found on the web.

Offering a molecular perspective not available 20 years ago, it provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents.

Just a small sampling of the wealth of information found inside the handbook:

• Buffers and buffer solutions
• Heat capacities and combustion levels
• Reagents for the chemical modification of proteins
• Comprehensive classification system for lipids
• Biological characteristics of vitamins
• A huge variety of UV data
• Recommendations for nomenclature and tables in biochemical thermodynamics
• Guidelines for NMR measurements for determination of high and low pka values
• Viscosity and density tables
• Chemical and physical properties of various commercial plastics
• Generic source-based nomenclature for polymers
• Therapeutic enzymes

About the Editors:

Roger L. Lundblad, Ph.D.

Roger L. Lundblad is a native of San Francisco, California. He received his undergraduate education at Pacific Lutheran University and his PhD degree in biochemistry at the University of Washington. After postdoctoral work in the laboratories of Stanford Moore and William Stein at the Rockefeller University, he joined the faculty of the University of North Carolina at Chapel Hill. He joined the Hyland Division of Baxter Healthcare in 1990. Currently Dr. Lundblad is an independent consultant and writer in biotechnology in Chapel Hill, North Carolina. He is an adjunct Professor of Pathology at the University of North Carolina at Chapel Hill and Editor-in-Chief of the Internet Journal of Genomics and Proteomics.

Fiona M. Macdonald, Ph.D., F.R.S.C.

Fiona M. Macdonald received her BSc in chemistry from Durham University, UK. She obtained her PhD in inorganic biochemistry at Birkbeck College, University of London, studying under Peter Sadler. Having spent most of her career in scientific publishing, she is now at Taylor and Francis and is involved in developing chemical information products.