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This digital document is an article from UXL Encyclopedia of Science, brought to you by Gale®, a part of Cengage Learning, a world leader in e-research and educational publishing for libraries, schools and businesses.The length of the article is 467 words.The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase.You can view it with any web browser.This collection of entries covers major areas of science including: biology, genetics, microbiology, astronomy, chemistry, physics, engineering, technology, geology, weather, archaeology, psychology, mathematics, and medicine, and provides readers with a wide range of up-to-date, relevant, and accurate information. ... Read more

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Webster's bibliographic and event-based timelines are comprehensive in scope, covering virtually all topics, geographic locations and people. They do so from a linguistic point of view, and in the case of this book, the focus is on "Cryobiology," including when used in literature (e.g. all authors that might have Cryobiology in their name). As such, this book represents the largest compilation of timeline events associated with Cryobiology when it is used in proper noun form. Webster's timelines cover bibliographic citations, patented inventions, as well as non-conventional and alternative meanings which capture ambiguities in usage. These furthermore cover all parts of speech (possessive, institutional usage, geographic usage) and contexts, including pop culture, the arts, social sciences (linguistics, history, geography, economics, sociology, political science), business, computer science, literature, law, medicine, psychology, mathematics, chemistry, physics, biology and other physical sciences. This "data dump" results in a comprehensive set of entries for a bibliographic and/or event-based timeline on the proper name Cryobiology, since editorial decisions to include or exclude events is purely a linguistic process. The resulting entries are used under license or with permission, used under "fair use" conditions, used in agreement with the original authors, or are in the public domain. ... Read more

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This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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Fish embryos have never been successfully cryopreserved because of the low permeability of cryoprotectants into the yolk. Recently, we used aquaporin-3 fused with a green fluorescent protein (AQP3GFP) to modify the zebrafish embryo, and demonstrated that the pores functioned physiologically. This increased the water and cryoprotectant permeability of the membranes. We have continued our work on AQP3-modified embryos and here we report their developmental expression of AQP3, the success of various culture media on their survival and development, and their reproductive success. The AQP3GFP expression begins within 30 m after the mRNA AQP3GFP injection into the yolk of the 1- to 4-cell embryo. This expression is distributed in the membranes throughout the blastoderm and the yolk syncytial layer within 24 h. It diminishes after 96 h. We found no difference in the survival or normal development of embryos from AQP3GFP or wild-type adults. Additionally, zebrafish embryos did not require special culture medium to survive after AQP3GFP modification. In fact, they survived best in embryo medium (ca. 40 mOsm). Embryos reared entirely in embryo medium had a higher percent survival and a higher percent normal development than those exposed to a high osmolality sucrose culture medium (ca. 330 mOsm). The mechanism whereby these embryos can maintain their internal osmolality in a hypoosmotic solution with water channels in their membranes is unknown. ... Read more

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This digital document is an article from Journal of the Alabama Academy of Science, published by Thomson Gale on April 1, 2006. The length of the article is 3392 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

Citation Details
Title: Alabama Academy of Science Symposium, 2006 "Southeastern Society for Environmental Toxicology and Chemistry" Annual Meeting, Troy University.(Conference notes)
Author: Gale Reference Team
Publication: Journal of the Alabama Academy of Science (Magazine/Journal)
Date: April 1, 2006
Publisher: Thomson Gale
Volume: 77Issue: 2Page: 120(9)

Article Type: Conference notes

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This digital document is an article from World of Microbiology and Immunology, brought to you by Gale®, a part of Cengage Learning, a world leader in e-research and educational publishing for libraries, schools and businesses.The length of the article is 756 words.The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase.You can view it with any web browser.Covers the concepts, theories, discoveries, and pioneers in microbiology and immunology, using a mix of traditional academic and topical articles, this title addresses current ethical, legal, and social issues with special emphasis given to biological warfare and terrorism. ... Read more

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This digital document is an article from Journal of the Alabama Academy of Science, published by Alabama Academy of Science on January 1, 2000. The length of the article is 336 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

Citation Details
Title: RESPONSE AND TOLERANCE OF Vibrio cholerae OI TO COLD TEMPERATURES.
Publication: Journal of the Alabama Academy of Science (Refereed)
Date: January 1, 2000
Publisher: Alabama Academy of Science
Page: 17

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Cryobiology is the study of living things at low temperatures. Refrigeration or freezing prevents the decay of living things, but science has recently begun to explore whether cold not only prevents decay, but also preserves life. An interdisciplinary subject that combines fundamental research on organisms adapted to natural freezing stresses and emerging areas of applied biotechnology and biology, cryobiology is starting to play an increasingly important role in medicine, agriculture, horticulture and forestry, as well as the conservation of endangered or economically important plant, animal and microbial species. Life in the Frozen State is a comprehensive review of the effects of freezing on living organisms.It evaluates how this knowledge can be applied to benefit humanity through advances in healthcare and conservation. A major feature of the book is the combining of fundamental theory and practice across a wide range of species and applications. This provides a unique opportunity to explore the subject in a multidisciplinary context. Historically, some of the most exciting advances in low temperature research have come from cross-discipline interaction, and this book provides a timely stimulus for further advances in cryobiology. ... Read more

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Cryotechniques offer a number of advantages over conventional electron microscopy methods in biology, particularly with regard to preserving the morphology and composition of specimens. This handbook provides a practical guide for researchers, students, and technicians who wish to employ cryotechniques in their work.It covers those methods where the cryofixed specimen is sectioned or otherwise presented as a thin film for examination, giving special attention to topics of current interest.This guide will be of use to workers in a variety of biomedical disciplines, including cell biology, molecular biology, bacteriology, and pathology. ... Read more

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In this latest volume in the series detailed practical guidance is given for the preparation of ultrathin sections for electron microscopy and of thicker sections for light microscopy from blocks of resin-embedded material. Techniques for the sectioning of the newer acrylic resins and the use of diamond knives are fully described, and methods for overcoming sectioning problems are discussed in detail. ... Read more